Canine Cystatin C ELISA Kit (ab273173)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 10.24 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine Cystatin C ELISA Kit
See all Cystatin C kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10 pg/ml -
Range
10.24 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106.3 89% - 115% Serum 96.03 87% - 105% Plasma 97.36 79% - 112% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog
Predicted to work with: Mouse
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Product overview
Canine Cystatin C ELISA Kit (ab273173) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine Cystatin C in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine Cystatin C coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine Cystatin C present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine Cystatin C antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine Cystatin C bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Canine Cystatin C coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine Cystatin C Antibody 2 vials Canine Cystatin C Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. -
Tissue specificity
Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. -
Involvement in disease
Amyloidosis 6
Macular degeneration, age-related, 11 -
Sequence similarities
Belongs to the cystatin family. -
Post-translational
modificationsThe Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AD 8
- AD8
- Amyloid angiopathy and cerebral hemorrhage
see all -
Database links
- Entrez Gene: 13010 Mouse
- SwissProt: P21460 Mouse
- Unigene: 4263 Mouse
Images
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Canine Cystatin C ELISA Kit standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.