Canine BDNF ELISA Kit (ab273265)
Key features and details
- Sensitivity: 0.5 pg/ml
- Range: 0.41 pg/ml - 100 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine BDNF ELISA Kit
See all BDNF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.5 pg/ml -
Range
0.41 pg/ml - 100 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 97.51 90% - 105% Plasma 94.91 68% - 122% Cell culture media 89.66 88% - 91% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Canine BDNF ELISA kit (ab273265) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine BDNF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine BDNF coated on a 96-well plate. Standards and samples are pipetted into the wells and BDNF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Canine BDNF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of BDNF bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Canine BDNF coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine BDNF Antibody 2 vials Canine BDNF Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. -
Tissue specificity
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. -
Involvement in disease
Bulimia nervosa 2
Congenital central hypoventilation syndrome -
Sequence similarities
Belongs to the NGF-beta family. -
Post-translational
modificationsThe propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG). -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Abrineurin
- ANON2
- BDNF
see all -
Database links
- Entrez Gene: 403461 Dog
- SwissProt: Q7YRB4 Dog
Images
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Example date
This standard curve is for demonstration only. A standard curve must be run with each assay.