Bradford Assay Kit (ab102535)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell culture extracts, Other biological fluids, Plasma, Serum, Tissue Extracts
Overview
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Product name
Bradford Assay Kit -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Assay time
0h 20m -
Product overview
Bradford Assay Kit ab102535 provides a simple and rapid procedure for determining the concentration of protein in solution.
The Bradford assay protocol uses an improved Coomassie blue G reagent which forms a blue complex in the presence of protein. The intensity of the blue complex is proportional to the amount of protein in the sample and can be easily measured by spectrophotometer or plate reader at 595 nm.
Bradford assay protocol summary:
- prepare serial dilutions of protein standard
- add samples and standards to wells or tubes
- add Bradford protein assay solution and incubate for 5 min at room temp
- measure absorbance at 595 nmSupport on how to determine protein concentration can be found here
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Notes
This product was previously called Protein Quantitation Kit (Bradford Assay).
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Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1000 tests Protein Assay Solution NM 1 x 20ml Protein Standard Yellow 1 x 1.5ml -
Research areas
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Relevance
Protein Quantitation is a procedure for determining the concentration of protein in solution.
Images
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Protein Assay - ab102535 Protein Quantification Kit (Bradford Assay)
Colorimetric standard curves of 150 microliter samples: mean of duplicates with background readings subtracted.
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Protein Assay - ab102535 Protein Quantitation Kit(Bradford Assay)Colorimetric standard curves of 100 microliter samples: mean of duplicates with background readings subtracted.
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BSA Standard CurveColorimetric standard curve of 10 microliter samples: mean of duplicates with background readings subtracted.