BCA protein assay kit reducing agent compatible (microplate) (ab207003)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Purified protein, Tissue Lysate
Overview
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Product name
BCA protein assay kit reducing agent compatible (microplate)
See all BCA Protein kits -
Detection method
Colorimetric -
Sample type
Cell Lysate, Purified protein, Tissue Lysate -
Product overview
Abcam’s BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
Unlike the majority of protein assay kits, ab207003 is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM and β-mercaptoethanol up to 35 mM. This kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu1+), which is generated by reduction of cupric cation (Cu2+) by the protein in alkaline conditions. The assay is linear over a wide range of protein concentrations (25-2000 μg/mL). The kit also includes Bovine Serum Albumin (BSA) as a reference protein standard. The kit has been formatted for microplate-based protein assays.
Support on how to determine protein concentration can be found here.
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Platform
Microplate reader
Properties
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Storage instructions
Store at room temperature. Please refer to protocols. -
Components 1000 tests BCA Reagent A 1 x 200ml BCA Reagent B 1 x 20ml Blocking Reagent 20 vials Blocking Reagent Buffer 1 x 20ml BSA 10 x 1ml -
Research areas
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Relevance
The BCA Protein assay (or Smith assay) is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is determined by measuring the color change of the sample solution from green to purple which is proportional to the protein concentration. -
Alternative names
- BCA
- Bicinchoninic acid
- Bicinchoninic acid protein
Images
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Standard Curve for BSA containing blocking reagent in the presence and absence of 10 mM DTT.
Standard curves for BSA containing blocking reagent in the presence and absence of 10 mM DTT obtained using ab207003.
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Standard Curve for BSA containing blocking reagent in the presence and absence of 20 mM TCEP.Standard curves for BSA containing blocking reagent in the presence and absence of 20 mM TCEP. Generated using ab207003.
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Standard Curve for BSA containing blocking reagent in the presence and absence of 35 mM ß-mercaptoethanol (BME).Standard curves for BSA containing blocking reagent in the presence and absence of 35 mM β-mercaptoethanol (BME). Generated using ab207003.
