BCA Protein Assay Kit (ab102536)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Assay time: 1 hr 30 min
- Sample type: Cell culture extracts, Plasma, Serum, Tissue Extracts
- Sensitivity: 0.5 µg
Overview
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Product name
BCA Protein Assay Kit
See all BCA Protein kits -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Cell culture extracts, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 0.5 µg -
Range
0.5 µg - 30 µg -
Assay time
1h 30m -
Product overview
BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.
The method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s).
The generated Cu2+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm.
The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 µg protein (0.01-0.6 mg/ml).
Support on how to determine protein concentration can be found here.
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Notes
This kit was previously called BCA Protein Quantification Kit.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1000 tests BCA Reagent NM 1 x 100ml BSA Standard Yellow 1 x 1ml Copper Reagent Blue 1 x 2ml -
Research areas
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Relevance
The BCA Protein assay (or Smith assay) is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is determined by measuring the color change of the sample solution from green to purple which is proportional to the protein concentration. -
Alternative names
- BCA
- Bicinchoninic acid
- Bicinchoninic acid protein
Images
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BCA Protein Assay using ab102536Standard Curve at 60°C.
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BCA Protein Assay using ab102536Standard Curve at 37°C.
