Biotin Anti-VEGF 165A antibody (ab271201)
Key features and details
- Biotin Goat polyclonal to VEGF 165A
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Overview
-
Product name
Biotin Anti-VEGF 165A antibody
See all VEGF 165A primary antibodies -
Description
Biotin Goat polyclonal to VEGF 165A -
Host species
Goat -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA HumanWB Recombinant fragment
-
Immunogen
Recombinant fragment corresponding to Human VEGF 165A aa 1-200.
Database link: P15692-4
Properties
-
Form
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Biotin Anti-VEGF 165A antibody (ab271201)
To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human VEGF165, respectively.
Non-reducing conditions.
-
Sandwich ELISA - Biotin Anti-VEGF 165A antibody (ab271201)Human VEGF165 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271201. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hVEGF protein.