Biotin Anti-IL-25 antibody (ab271280)
Key features and details
- Biotin Rabbit polyclonal to IL-25
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-IL-25 antibody
See all IL-25 primary antibodies -
Description
Biotin Rabbit polyclonal to IL-25 -
Host species
Rabbit -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA Recombinant fragmentWB Recombinant fragment
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Immunogen
Recombinant fragment corresponding to Human IL-25 aa 1 to the C-terminus. E. coli derived.
Database link: Q9H293 -
Positive control
- WB: Recombinant human IL-25.
Properties
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Form
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Biotin Anti-IL-25 antibody (ab271280)
Lane 1: Marker.
Lane 2: Recombinant hIL-25 at 250ng/lane.
Lane 3: Recombinant hIL-25 at 125ng/lane.
Lane 4: Recombinant hIL-25 at 62.5ng/lane.
Lane 5: Recombinant hIL-25 at 31.25ng/lane.
Lane 6: Recombinant hIL-25 at 15.625ng/lane.
Lane 7: Recombinant hIL-25 at 7.8ng/lane.
Lane 8: Recombinant hIL-25 at 3.9ng/lane.
Lane 9: Recombinant hIL-25 at 1.95ng/lane.
Lane 10: Recombinant hIL-25 at 0.975ng/lane.
Lane 11: Recombinant hIL-25 at 0.4875ng/lane.
Lane 12: Recombinant hIL-25 at 0.24ng/lane. -
Sandwich ELISA - Biotin Anti-IL-25 antibody (ab271280)Human IL-17E (IL-25) was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271280. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IL-17E protein.