Biotin Anti-Fluorescein antibody (ab6655)
Key features and details
- Biotin Goat polyclonal to Fluorescein
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, In situ hybridization, WB
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Fluorescein antibody
See all Fluorescein primary antibodies -
Description
Biotin Goat polyclonal to Fluorescein -
Host species
Goat -
Conjugation
Biotin -
Tested applications
Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, In situ hybridization, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Fluorescein conjugated to Goat IgG
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General notes
Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Biotin Anti-Fluorescein antibody (ab6655)Biotin Anti-Fluorescein antibody (ab6655) at 1/1000 dilution + BSA conjugated Fluorescein.
Secondary
Peroxidase conjugated Streptavidin secondary antibody at 1/40000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fluorescein antibody (ab6655) Cropped image from Long et al, Nature Communications, 7, 10354, Fig. 7a.; doi: 10.1038/ncomms10354 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemical analysis of chicken embryo neuroepithelium labelling fluorescein-tagged morpholinos with ab6655. Samples were fixed in 4% paraformaldehyde at 4°C for 1 hour.
