Biotin Anti-BrdU antibody (ab2284)
Key features and details
- Biotin Sheep polyclonal to BrdU
- Suitable for: IHC-P, IHC-Fr, ICC/IF, ELISA, IHC-FoFr
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-BrdU antibody
See all BrdU primary antibodies -
Description
Biotin Sheep polyclonal to BrdU -
Host species
Sheep -
Conjugation
Biotin -
Tested applications
Suitable for: IHC-P, IHC-Fr, ICC/IF, ELISA, IHC-FoFrmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituent: 0.4% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (ab2284) This image is courtesy of an anonymous Abreview
ab2284 staining BrdU in mouse intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% H2O2 in methanol for 12 minutes; antigen retrieval was by heat mediation in 10mM citrate, pH6. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C.
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Immunocytochemistry/ Immunofluorescence - Biotin Anti-BrdU antibody (ab2284) This image is courtesy of an anonymous Abreviewab2284 at 1/250 staining primary E12 mouse cortex cells by ICC/IF. The cells were paraformaldehyde fixed, blocked with serum and then incubated with the antibody for 24 hours. Streptavidin conjugated to Alexa-Fluor ® 488 was used as the secondary. The image shows BrdU staining with nuclei counterstained with DAPI.
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Immunohistochemistry (Frozen sections) - Biotin Anti-BrdU antibody (ab2284) This image is courtesy of an Abreview submitted by Ahmar Azizab2284 staining BrdU in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 30 minutes. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 4°C. A Streptavidin Alexa Fluor® 488-conjugated Goat polyclonal (1/500) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (ab2284) This image is courtesy of an Abreview submitted by Dr Christoph Schwarzerab2284 at 1/2000 dilution staining mouse free floating brain slices by Immunohistochemistry (Formalin/PFA fixed sections). The mice were treated with 100mg/kg BrdU 2 hours before fixation. Free floating 40µm vibratome sections were obtained from paraformaldehyde fixed brains, these were incubated with the antibody for 24 hours. A streptavidin-HRP complex and DAB were used for detection. The image depicts the subventricular zone.
