Beta-Xylosidase Activity Assay Kit (Fluorometric) (ab273305)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Flow cytometry-fluorescent
- Platform: Microplate
Overview
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Product name
Beta-Xylosidase Activity Assay Kit (Fluorometric) -
Detection method
Flow cytometry-fluorescent -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Beta-Xylosidase Activity Assay Kit (Fluorometric) (ab273305) provides a quick, reliable, and sensitive fluorometric method for the determination of Beta-Xylosidase activity. A fluorogenic substrate is incubated with the sample, and in the presence of Beta-Xylosidase activity, the substrate is cleaved, releasing a fluorophore (Ex/Em 330/450 nm).
When used according to protocol, activities as low as one µUnit (1 pmol/min) in environmental microbial samples, such as compost, can be detected.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Beta-Xylosidase Assay Buffer 1 x 25ml Beta-Xylosidase Positive Control (Lyophilized) 1 vial Beta-Xylosidase Substrate (in DMSO) 1 x 250µl 4-Methylumbelliferone Standard (5 mM) 1 x 35µl
Images
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Example standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Time course using positive control ß-Xylosidase as described in Sample Preparation;Example Data.
Time course using positive control Beta-Xylosidase as described in Sample Preparation.
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Determination of beta xylosidase activity in compost samplesExample of determination of Beta-Xylosidase activity in compost sample. Compost (10 g) was incubated for 48 hrs at room temperature in tap water (10 ml), at which point the resulting broth was clarified by centrifugation. Rates were determined by incubation of given volumes of resultant broth with Beta-Xylosidase Substrate as described for 2 hours.
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Determination of Beta-Xylosidase activity in compost samplesExample Data