BAPTA-AM, Ca2+ chelator (ab120503)
Key features and details
- Selective Ca2+ chelator. Analog of BAPTA.
- CAS Number: 126150-97-8
- Purity: > 97%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
BAPTA-AM, Ca2+ chelator -
Description
Selective Ca2+ chelator. Analog of BAPTA. -
Biological description
Selective Ca2+ chelator. Cell-permeable analog of BAPTA (ab120449). Useful for manipulation of intracellular free Ca2+ levels. Shows varied biological activity. Blocks hKv1.5, Kv11.1 (hERG) and hKv1.3 channels (Ki values are 1.23, 1.30 and 1.45 μM, respectively).
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Purity
> 97% -
CAS Number
126150-97-8 -
Chemical structure
Images
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Functional Studies - BAPTA-AM, Ca2+ chelator (ab120503)ab66705 staining PAI1 in HepG2 cells treated with BAPTA-AM (ab120503), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of BAPTA-AM, as described in literature.
The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120503 (BAPTA-AM) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
