Arginase Activity Assay Kit (Colorimetric) (ab180877)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.2 U/ml
Overview
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Product name
Arginase Activity Assay Kit (Colorimetric)
See all Liver Arginase kits -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Sensitivity
Assay time
1h 00mSpecies reactivity
Reacts with: Mammals, Other speciesProduct overview
Arginase Activity Assay Kit (Colorimetric) ab180877 is a simple, sensitive and rapid assay to quantify arginase activity.
In the arginase assay protocol, arginase reacts with arginine and undergoes a series of reactions that form an intermediate that react stoichiometrically with the probe to generate a colored product that can be detected at OD = 570 nm.
The kit can detect less than 0.2 U/L Arginase activity in 96-well assay format.
Arginase assay protocol summary:
- add samples and standards to wells
- add arginase substrate mix to sample wells only
- incubate for 20 min
- add arginase assay reaction mix to all wells
- analyze with microplate reader in kinetic mode for 10-30 minNotes
Arginase (EC 3.5.3.1) is the final enzyme of the Urea Cycle. It converts L-arginine into urea and L-ornithine and plays an important role in removing ammonium ion from the body.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Arginase Assay Buffer WM 1 x 25ml Arginase Converter Enzyme (Lyophilized) Blue 1 vial Arginase Developer (Lyophilized) Orange 1 vial Arginase Enzyme Mix (Lyophilized) Green 1 vial Arginase Positive Control (Lyophilized) Purple 1 vial Arginase Substrate (Lyophilized) White 1 vial Hydrogen peroxide Yellow 1 x 100µl OxiRed Probe in DMSO Red 1 x 200µl -
Research areas
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Pathway
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
Involvement in disease
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. -
Sequence similarities
Belongs to the arginase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- A I
- Al
- ARG 1
see all
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Arginase Assay Buffer WM 1 x 25ml Arginase Converter Enzyme (Lyophilized) Blue 1 vial Arginase Developer (Lyophilized) Orange 1 vial Arginase Enzyme Mix (Lyophilized) Green 1 vial Arginase Positive Control (Lyophilized) Purple 1 vial Arginase Substrate (Lyophilized) White 1 vial Hydrogen peroxide Yellow 1 x 100µl OxiRed Probe in DMSO Red 1 x 200µl -
Research areas
-
Pathway
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
Involvement in disease
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. -
Sequence similarities
Belongs to the arginase family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Alternative names
- A I
- Al
- ARG 1
see all
Images
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H202 Standard Curve
H2O2 Standard Curve. Assay performed following kit protocol.
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Arginase activityArginase activity in rat liver lysate (3 µg) & Positive Control (2 µL). Assays were performed following the kit protocol.