Anti-GALNT1 antibody (ab109918)
Key features and details
- Rabbit polyclonal to GALNT1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GALNT1 antibody
See all GALNT1 primary antibodies -
Description
Rabbit polyclonal to GALNT1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in both Human Kidney and Lung tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab109918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB 1/250. Detects a band of approximately 59 kDa (predicted molecular weight: 64 kDa). Target
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Function
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b and Muc7. -
Tissue specificity
Widely expressed. Expressed in all tissues tested. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
Contains 1 ricin B-type lectin domain. -
Domain
There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
The ricin B-type lectin domain directs the glycopeptide specificity. It is required in the glycopeptide specificity of enzyme activity but not for activity with naked peptide substrates, suggesting that it triggers the catalytic domain to act on GalNAc-glycopeptide substrates. -
Cellular localization
Secreted and Golgi apparatus > Golgi stack membrane. - Information by UniProt
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Database links
- Entrez Gene: 2589 Human
- Entrez Gene: 14423 Mouse
- Entrez Gene: 79214 Rat
- Omim: 602273 Human
- SwissProt: Q10472 Human
- SwissProt: O08912 Mouse
- SwissProt: Q10473 Rat
- Unigene: 514806 Human
see all -
Alternative names
- GALNAC T1 antibody
- GalNAc transferase 1 antibody
- GalNAc-T1 antibody
see all
Images
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Western blot - Anti-GALNT1 antibody (ab109918)All lanes : Anti-GALNT1 antibody (ab109918) at 1/250 dilution
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Exposure time: 20 minutes
We hypothesize that the band observed at 59 kDa represents the soluble form of GALNT1 due to the presence of a 40 amino acid signal peptide. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab109918 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab109918 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-GALNT1 antibody (ab109918)All lanes : Anti-GALNT1 antibody (ab109918) at 1/250 dilution
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Exposure time: 20 minutes
We hypothesize that the band observed at 59 kDa represents the soluble form of GALNT1 due to the presence of a 40 amino acid signal peptide. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab109918 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.