Anti-P2X4 antibody (ab134559)
Key features and details
- Goat polyclonal to P2X4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-P2X4 antibody
See all P2X4 primary antibodies -
Description
Goat polyclonal to P2X4 -
Host species
Goat -
Specificity
Preliminary testing was unsuccessful on Mouse and Rat Kidney and Liver in Western blot.
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Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Pig, Orangutan
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Immunogen
Synthetic peptide corresponding to Mouse P2X4 aa 56-69 (internal sequence) (Cysteine residue).
Sequence:ETDSVVSSVTTKAK
Database link: Q9JJX6 -
Positive control
- WB: A549 cell lysate; ICC/IF: A431 cells; IHC-P: Human uterus tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-P2X4 antibody (ab134559)Anti-P2X4 antibody (ab134559) at 0.3 µg/ml + A549 (human lung carcinoma cell line) cell lysate (35µg protein in RIPA buffer)
Predicted band size: 43 kDa
Observed band size: 48-50 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-P2X4 antibody (ab134559)Immunofluorescence analysis of paraformaldehyde fixed A431 cells labeling P2X4 with ab134559. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (4 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (4 µg/mL).
