Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZWINT antibody [EPR23440-96] (ab252950)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23440-96] to ZWINT
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-ZWINT antibody [EPR23440-96]
    See all ZWINT primary antibodies

  • Description

    Rabbit monoclonal [EPR23440-96] to ZWINT

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji, MOLT-4, PC-3, NIH:OVCAR-3, SW480, A549, HeLa and A431 whole cell lysates. IHC-P: Human testis tissue and lung cancer tissue. Flow Cyt: HeLa and MOLT-4 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ZWINT antibody [EPR23440-96] (ab252950)
    Western blot - Anti-ZWINT antibody [EPR23440-96] (ab252950)
    All lanes : Anti-ZWINT antibody [EPR23440-96] (ab252950) at 1/1000 dilution

    Lane 1 : Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate at 20 µg
    Lane 2 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate at 20 µg
    Lane 3 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 4 : NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 5 : SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 6 : A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg
    Lane 7 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 8 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 31 kDa
    Observed band size: 30,36 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The positive bands are consistent with two isoforms and literature (PMID:23979716, 29440916).

    Exposure time: 3 minutes.

  • Flow Cytometry - Anti-ZWINT antibody [EPR23440-96] (ab252950)
    Flow Cytometry - Anti-ZWINT antibody [EPR23440-96] (ab252950)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling ZWINT with ab252950 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-ZWINT antibody [EPR23440-96] (ab252950)
    Flow Cytometry - Anti-ZWINT antibody [EPR23440-96] (ab252950)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ZWINT with ab252950 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZWINT antibody [EPR23440-96] (ab252950)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZWINT antibody [EPR23440-96] (ab252950)

    Immunohistochemical analysis of paraffin-embedded human lung cancer (Panel A) and its adjacent noncancerous tissue (Panel B) tissue labeling ZWINT with ab252950 at 1/250 dilution (1.784 ug/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human lung cancer tissue is observed (Panel A) while neagtive staining is observed in its adjacent non-cancerous tissue (Panel B) (PMID: 29615843). The section was incubated with ab252950 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZWINT antibody [EPR23440-96] (ab252950)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZWINT antibody [EPR23440-96] (ab252950)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling ZWINT with ab252950 at 1/250 dilution (1.784 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human testis tissue is observed (PMID: 22023800). The section was incubated with ab252950 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-ZWINT antibody [EPR23440-96] (ab252950)
    Anti-ZWINT antibody [EPR23440-96] (ab252950)

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