Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

Order now and get it on Wednesday March 10, 2021

Anti-ZWINT antibody [EPR23440-96] (ab252950)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23440-96] to ZWINT
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-ZWINT antibody [EPR23440-96]
See all ZWINT primary antibodies
Description

Rabbit monoclonal [EPR23440-96] to ZWINT
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Raji, MOLT-4, PC-3, NIH:OVCAR-3, SW480, A549, HeLa and A431 whole cell lysates. IHC-P: Human testis tissue and lung cancer tissue. Flow Cyt: HeLa and MOLT-4 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23440-96
Isotype

IgG
Research areas

Western blot - Anti-ZWINT antibody [EPR23440-96] (ab252950)

All lanes : Anti-ZWINT antibody [EPR23440-96] (ab252950) at 1/1000 dilution

Lane 1 : Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate at 20 µg
Lane 2 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate at 20 µg
Lane 3 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 4 : NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 5 : SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 6 : A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg
Lane 7 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 8 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 31 kDa
Observed band size: 30,36 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The positive bands are consistent with two isoforms and literature (PMID:23979716, 29440916).

Exposure time: 3 minutes.
Flow Cytometry - Anti-ZWINT antibody [EPR23440-96] (ab252950)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling ZWINT with ab252950 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry - Anti-ZWINT antibody [EPR23440-96] (ab252950)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ZWINT with ab252950 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZWINT antibody [EPR23440-96] (ab252950)

Immunohistochemical analysis of paraffin-embedded human lung cancer (Panel A) and its adjacent noncancerous tissue (Panel B) tissue labeling ZWINT with ab252950 at 1/250 dilution (1.784 ug/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human lung cancer tissue is observed (Panel A) while neagtive staining is observed in its adjacent non-cancerous tissue (Panel B) (PMID: 29615843). The section was incubated with ab252950 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZWINT antibody [EPR23440-96] (ab252950)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling ZWINT with ab252950 at 1/250 dilution (1.784 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human testis tissue is observed (PMID: 22023800). The section was incubated with ab252950 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-ZWINT antibody [EPR23440-96] (ab252950)

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