Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZWILCH antibody [EPR18035-28] (ab202898)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18035-28] to ZWILCH
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ZWILCH antibody [EPR18035-28]
    See all ZWILCH primary antibodies

  • Description

    Rabbit monoclonal [EPR18035-28] to ZWILCH

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, HeLa, PC-3, MCF7, C6, PC-12 and NIH/3T3 cell lysates; Mouse brain lysate. IP: HeLa whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18035-28

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
    Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
    All lanes : Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution

    Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) cell lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 15 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
    Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
    Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution + Mouse brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
    Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
    All lanes : Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cells) cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate
    Lane 3 : NIH/3T3 (Mouse embryo fibroblast cells) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 15 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-ZWILCH antibody [EPR18035-28] (ab202898)
    Immunoprecipitation - Anti-ZWILCH antibody [EPR18035-28] (ab202898)

    ZWILCH was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab202898 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab202898 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. 

    Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab202898 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202898 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-ZWILCH antibody [EPR18035-28] (ab202898)
    Anti-ZWILCH antibody [EPR18035-28] (ab202898)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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