Anti-ZEB1 antibody [2A8A6] (ab181451)
![Anti-ZEB1 antibody [2A8A6] (ab181451)](https://www.abcam.com/ps/products/181/ab181451/Images/ab181451-298747-anti-zeb1-antibody-2a8a6-western-blot.jpg "Anti-ZEB1 antibody [2A8A6] (ab181451)")
Key features and details
- Mouse monoclonal [2A8A6] to ZEB1
- Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-ZEB1 antibody [2A8A6]
See all ZEB1 primary antibodies -
Description
Mouse monoclonal [2A8A6] to ZEB1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB HumanRecombinant fragment
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Immunogen
Recombinant fragment corresponding to Human ZEB1 aa 967-1108. Expressed in E. coli.
Sequence:KRFS HSGSYSQHMN HRYSYCKREA EERDSTEQEE AGPEILSNEH VGARASPSQG DSDERESLTR EEDEDSEKEE EEEDKEMEEL QEEKECEKPQ GDEEEEEEEE EVEEEEVEEA ENEGEEAKTE GLMKDDRAES QASSLGQK
Database link: P37275 -
Positive control
- WB: Human ZEB1 recombinant protein and HAP1 cell lysate. IHC-P: Human cervical cancer and rectum cancer tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR224480-10 are from Tissue Culture Supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
0.5% protein stabilizer consisting of amino acid =85% pH(1% water solution) =7.0~7.5 Water =2% As(mg/kg) =0.5 Pb(mg/kg) =0.1 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2A8A6 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-ZEB1 antibody [2A8A6] (ab181451)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ZEB1 knockout HAP1 whole cell lysate (20 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab181451 observed at 200 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab181451 was shown to recognize ZEB1 in wild-type HAP1 cells as signal was lost at the expected MW in ZEB1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ZEB1 knockout samples were subjected to SDS-PAGE. Ab181451 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ZEB1 antibody [2A8A6] (ab181451)](https://www.abcam.com/ps/products/181/ab181451/Images/ab181451-211157-anti-zeb1-antibody-2a8a6-western-blot.jpg)
Western blot - Anti-ZEB1 antibody [2A8A6] (ab181451)All lanes : Anti-ZEB1 antibody [2A8A6] (ab181451) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : KEB1 (AA: 967-1108)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 124 kDa
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![Western blot - Anti-ZEB1 antibody [2A8A6] (ab181451)](https://www.abcam.com/ps/products/181/ab181451/Images/ab181451-211156-anti-zeb1-antibody-2a8a6-western-blot.jpg)
Western blot - Anti-ZEB1 antibody [2A8A6] (ab181451)Anti-ZEB1 antibody [2A8A6] (ab181451) at 1/500 dilution + ZEB1 recombinant protein fragment (Expected MW is 41.7 kDa)
Predicted band size: 124 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [2A8A6] (ab181451)](https://www.abcam.com/ps/products/181/ab181451/Images/ab181451-211155-anti-zeb1-antibody-2a8a6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [2A8A6] (ab181451)Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling ZEB1 with ab181451 at 1/200 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [2A8A6] (ab181451)](https://www.abcam.com/ps/products/181/ab181451/Images/ab181451-211153-anti-zeb1-antibody-2a8a6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [2A8A6] (ab181451)Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling ZEB1 with ab181451 at 1/200 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody [2A8A6] (ab181451)](https://www.abcam.com/ps/products/181/ab181451/Images/ab181451-211152-anti-zeb1-antibody-2a8a6-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody [2A8A6] (ab181451)Immunofluorescence analysis of HeLa cells labeling ZEB1 (green) with ab181451 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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![Flow Cytometry - Anti-ZEB1 antibody [2A8A6] (ab181451)](https://www.abcam.com/ps/products/181/ab181451/Images/ab181451-211150-anti-zeb1-antibody-2a8a6-flow-cytometry.jpg)
Flow Cytometry - Anti-ZEB1 antibody [2A8A6] (ab181451)Flow cytometric analysis of HeLa cells labeling ZEB1 (green) with ab181451 at 1/200 dilution. Negative control (red).
