Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Zic1 antibody [EPR7291(2)] (ab134951)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7291(2)] to Zic1
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Zic1 antibody [EPR7291(2)]
    See all Zic1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7291(2)] to Zic1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide corresponding to residues within Human Zic1 (UniProt ID: Q15915).

  • Positive control

    • Human cerebellum lysate, Human brain, Human fetal brain lysate, SH SY5Y cell lysate, SW480 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
    Western blot - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
    All lanes : Anti-Zic1 antibody [EPR7291(2)] (ab134951) at 1/2000 dilution (purified)

    Lane 1 : Human brain lysates
    Lane 2 : Human cerebellum lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 48 kDa



    Blocking and diluting buffer: 5% NFDM/TBST. 

  • Flow Cytometry - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
    Flow Cytometry - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
    Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Zic1 with purified ab134951 at 1:70 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry/ Immunofluorescence - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
    Immunocytochemistry/ Immunofluorescence - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
    Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Zic1  with Purified ab134951 at 1:200 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
    Flow Cytometry - Anti-Zic1 antibody [EPR7291(2)] (ab134951)

    Overlay histogram showing SH-SY5Y cells stained with unpurified ab134951 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134951, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
    Western blot - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
    All lanes : Anti-Zic1 antibody [EPR7291(2)] (ab134951) at 1/1000 dilution (unpurified)

    Lane 1 : Human cerebellum tissue lysate
    Lane 2 : Human fetal brain tissue lysate
    Lane 3 : SH SY5Y cell lysate
    Lane 4 : SW480 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 48 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Zic1 antibody [EPR7291(2)] (ab134951)
    Immunocytochemistry/ Immunofluorescence - Anti-Zic1 antibody [EPR7291(2)] (ab134951)

    Immunofluorescent staining of SW480 cells labelling Zic1 with unpurified ab134951 at 1/100 dilution

  • Anti-Zic1 antibody [EPR7291(2)] (ab134951)
    Anti-Zic1 antibody [EPR7291(2)] (ab134951)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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