Anti-ZCCHC6 antibody (ab76901)
Key features and details
- Mouse polyclonal to ZCCHC6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZCCHC6 antibody
See all ZCCHC6 primary antibodies -
Description
Mouse polyclonal to ZCCHC6 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Full length human ZCCHC6 protein (aa 1-412 of AAH32456 or Swissprot id Q5VYS8-5).
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Positive control
- Human liver tissue lysate; ZCCHC6 transfected 293T cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ZCCHC6 antibody (ab76901)Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml + human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 171 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
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Western blot - Anti-ZCCHC6 antibody (ab76901)All lanes : Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml
Lane 1 : ZCCHC6 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 171 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Note: predicted molecular weight for isoform 5 is 48 kDa. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC6 antibody (ab76901)ab76901 staining ZCCHC6 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent antigen retrieval in 0.1 sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining method was used for detection.
