Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13121-79] to RBPMS2 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free
    See all RBPMS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13121-79] to RBPMS2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250345 is the carrier-free version of ab181098. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250345 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)
    All lanes : Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 2000 µg (purified)

    Lane 1 : PC-3 lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Human fetal liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa
    Additional bands at: 24 kDa (possible isoform), 26 kDa (possible isoform)



    This data was developed using ab181098, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)
    Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (purified) + Mouse heart tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab181098, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)
    Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (purified) + Rat heart tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa
    Additional bands at: 24 kDa (possible isoform), 26 kDa (possible isoform)



    This data was developed using ab181098, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)
    Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)
    This data was developed using ab181098, the same antibody clone in a different buffer formulation.ab181098 (purified) at 1/50 immunoprecipitating RBPMS2 in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.
  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)
    All lanes : Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (unpurified)

    Lane 1 : PC3 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa



    This data was developed using ab181098, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)
    Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (unpurified) + Human fetal liver lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 22 kDa



    This data was developed using ab181098, the same antibody clone in a different buffer formulation.

     

  • Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)
    Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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