Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13121-79] to RBPMS2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free
See all RBPMS2 primary antibodies -
Description
Rabbit monoclonal [EPR13121-79] to RBPMS2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250345 is the carrier-free version of ab181098. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250345 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13121-79 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 2000 µg (purified)
Lane 1 : PC-3 lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Human fetal liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 22 kDa
Additional bands at: 24 kDa (possible isoform), 26 kDa (possible isoform)This data was developed using ab181098, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (purified) + Mouse heart tissue lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?This data was developed using ab181098, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (purified) + Rat heart tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 22 kDa
Additional bands at: 24 kDa (possible isoform), 26 kDa (possible isoform)This data was developed using ab181098, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab181098, the same antibody clone in a different buffer formulation.ab181098 (purified) at 1/50 immunoprecipitating RBPMS2 in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (unpurified)
Lane 1 : PC3 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDaThis data was developed using ab181098, the same antibody clone in a different buffer formulation.
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Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (unpurified) + Human fetal liver lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 22 kDaThis data was developed using ab181098, the same antibody clone in a different buffer formulation.
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