Antibodies, Proteins, Kits and Reagents for Life Science

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Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4652] to YY1 - Nuclear Loading Control
Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-YY1 antibody [EPR4652] - Nuclear Loading Control
See all YY1 primary antibodies
Description

Rabbit monoclonal [EPR4652] to YY1 - Nuclear Loading Control
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Purchase matching WB positive control:Recombinant Human YY1 protein
- WB: HeLa, Daudi, Y79, and HuT-78 cell lysates, mouse and rat heart tissue. IHC-P: Human kidney, tonsil and cervix carcinoma tissues. ICC/IF: HeLa and HUT-78 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4652
Isotype

IgG
Research areas

Western blot - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/10000 dilution (purified)

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma cell line) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 45 kDa
Observed band size: 68 kDa
why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling YY1 with purified ab109237 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Flow Cytometry - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

ab109237 staining YY1 in the human cell line HeLa (Human epithelial cell line from cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/30. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control: Rabbit monoclonal IgG (Black).

Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

Immunocytochemistry/Immunofluorescence analysis of HUT-78 cells labelling YY1 with purified ab109237 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).
Western blot - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/50000 dilution (purified)

Lane 1 : Y79 (Human retinoblastoma cell line) cell lysate
Lane 2 : HuT-78 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 45 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/2000 dilution (purified)

Lane 1 : Mouse heart
Lane 2 : Rat heart

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 45 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/1000 dilution (unpurified)

Lane 1 : Daudi (Human Burkitt's lymphoma cell line) cell lysate
Lane 2 : Y79 (Human retinoblastoma cell line) cell lysate
Lane 3 : HuT-78 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 45 kDa
Immunocytochemistry/ Immunofluorescence - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling YY1 with unpurified ab109237 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human tonsil tissue labelling YY1 with unpurified ab109237 at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labelling YY1 with unpurified ab109237 at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Images

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