Anti-YY1 antibody [EPR4651] (ab109228)
![Anti-YY1 antibody [EPR4651] (ab109228)](https://www.abcam.com/ps/products/109/ab109228/Images/ab109228-121895-anti-yy1-antibody-epr4651-western-blot.jpg "Anti-YY1 antibody [EPR4651] (ab109228)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4651] to YY1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-YY1 antibody [EPR4651]
See all YY1 primary antibodies -
Description
Rabbit monoclonal [EPR4651] to YY1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Purchase matching WB positive control:Recombinant Human YY1 protein
- Human colon tissue; HeLa, Y79 and HuT-78 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4651 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-YY1 antibody [EPR4651] (ab109228)All lanes : Anti-YY1 antibody [EPR4651] (ab109228) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Y79 cell lysates
Lane 3 : HuT-78 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4651] (ab109228)](https://www.abcam.com/ps/products/109/ab109228/Images/ab109228-121894-anti-yy1-antibody-epr4651-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4651] (ab109228)Immunohistochemical analysis of YY1 in paraffin embedded Human colon tissue, using a 1/250 dilution of ab109228.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-YY1 antibody [EPR4651] (ab109228)](https://www.abcam.com/ps/products/109/ab109228/Images/ab109228-3-benefits-of-recombinant-antibodies.png)
Anti-YY1 antibody [EPR4651] (ab109228)
