Anti-YTHDC2 antibody (ab176846)
Key features and details
- Rabbit polyclonal to YTHDC2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-YTHDC2 antibody
See all YTHDC2 primary antibodies -
Description
Rabbit polyclonal to YTHDC2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human YTHDC2 aa 1200-1250. The exact sequence is proprietary. NP_073739.3
Sequence:K SSADTEFSDE CTTAERVLMK SPSPALHPPQ KYKDRGILHP KRGTEDRSDQ
Database link: Q9H6S0 -
Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176846 was affinity purified using an epitope specific to YTHDC2 immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-YTHDC2 antibody (ab176846)All lanes : Anti-YTHDC2 antibody (ab176846) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 5 µg
Lane 4 : 293T whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 160 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-YTHDC2 antibody (ab176846)Detection of YTHDC2 by Western Blot of Immunoprecipitate.
ab176846 at 0.4 µg/ml labeling YTHDC2 in HeLa whole cell lysate immunoprecipitated using ab176846 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).Detection: Chemiluminescence with exposure time of 10 seconds.
