Anti-WRNIP1/WHIP antibody (ab99316)
Key features and details
- Rabbit polyclonal to WRNIP1/WHIP
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WRNIP1/WHIP antibody
See all WRNIP1/WHIP primary antibodies -
Description
Rabbit polyclonal to WRNIP1/WHIP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide corresponding to Human WRNIP1/WHIP aa 400-450.
Database link: NP_064520.2 -
Positive control
- HeLa whole cell lysate
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General notes
This product was previously labelled as WRNIP1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab99316 was affinity purified using an epitope specific to WRNIP1/WHIP immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-WRNIP1/WHIP antibody (ab99316) at 1/1000 dilution + Mouse T cell lysate at 80 µg
Secondary
IRDye® 680-conjugated Goat anti-rabbit IgG polyclonal at 1/4000 dilution
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling WRNIP1/WHIP with ab99316 at 1/1000 (1µg/ml). Detection: DAB.
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All lanes : Anti-WRNIP1/WHIP antibody (ab99316) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 30 seconds
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Detection of WRNIP1/WHIP by Western Blot of Immunprecipitate.
ab99316, at 1 µg/ml, staining WRNIP1/WHIP in HeLa whole cell lysate immunoprecipitated using ab99316 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.