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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-WRNIP1/WHIP antibody (ab99316)

Anti-WRNIP1/WHIP antibody (ab99316)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to WRNIP1/WHIP
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-WRNIP1/WHIP antibody
    See all WRNIP1/WHIP primary antibodies
  • Description

    Rabbit polyclonal to WRNIP1/WHIP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human WRNIP1/WHIP aa 400-450.
    Database link: NP_064520.2

  • Positive control

    • HeLa whole cell lysate
  • General notes

     This product was previously labelled as WRNIP1

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab99316 was affinity purified using an epitope specific to WRNIP1/WHIP immobilized on solid support
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Images

  • Western blot - Anti-WRNIP1/WHIP antibody (ab99316)
    Western blot - Anti-WRNIP1/WHIP antibody (ab99316) This image is courtesy of an anonymous Abreview
    Anti-WRNIP1/WHIP antibody (ab99316) at 1/1000 dilution + Mouse T cell lysate at 80 µg

    Secondary
    IRDye® 680-conjugated Goat anti-rabbit IgG polyclonal at 1/4000 dilution

    Performed under reducing conditions.

    Predicted band size: 69 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WRNIP1/WHIP antibody (ab99316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WRNIP1/WHIP antibody (ab99316)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling WRNIP1/WHIP with ab99316 at 1/1000 (1µg/ml). Detection: DAB.

  • Western blot - Anti-WRNIP1/WHIP antibody (ab99316)
    Western blot - Anti-WRNIP1/WHIP antibody (ab99316)
    All lanes : Anti-WRNIP1/WHIP antibody (ab99316) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 69 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation - Anti-WRNIP1/WHIP antibody (ab99316)
    Immunoprecipitation - Anti-WRNIP1/WHIP antibody (ab99316)

    Detection of WRNIP1/WHIP by Western Blot of Immunprecipitate.
    ab99316, at 1 µg/ml, staining WRNIP1/WHIP in HeLa whole cell lysate immunoprecipitated using ab99316 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: IHC-P, WB

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    Anti-WRNIP1/WHIP antibody (ab4731)

    Applications: WB

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