Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDE4B antibody [EPR11830] (ab170939)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11830] to PDE4B
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PDE4B antibody [EPR11830]
    See all PDE4B primary antibodies

  • Description

    Rabbit monoclonal [EPR11830] to PDE4B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human PDE4B aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: Q07343

  • Positive control

    • WB: HeLa, A549, Human fetal brain and Human glioma lysates; Mouse brain tissue lysate. Flow Cyt: A549 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11830

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)
    Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)
    All lanes : Anti-PDE4B antibody [EPR11830] (ab170939) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PDE4B knockout HeLa cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab170939 observed at 83 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab170939 Anti-PDE4B antibody [EPR11830] was shown to specifically react with PDE4B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265259 (knockout cell lysate ab257576) was used.  Wild-type and PDE4B knockout samples were subjected to SDS-PAGE.  ab170939 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)
    Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)
    All lanes : Anti-PDE4B antibody [EPR11830] (ab170939) at 1/1000 dilution

    Lane 1 : Human fetal brain lysates
    Lane 2 : Human glioma lysates
    Lane 3 : A549 lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 83 kDa

  • Flow Cytometry - Anti-PDE4B antibody [EPR11830] (ab170939)
    Flow Cytometry - Anti-PDE4B antibody [EPR11830] (ab170939)

    Flow cytometric analysis of permeabilized A549 cells labeling PDE4B using ab170939 (red) at 1/10 dilution, or a rabbit IgG (negative) (green).

  • Anti-PDE4B antibody [EPR11830] (ab170939)
    Anti-PDE4B antibody [EPR11830] (ab170939)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PDE4B antibody [EPR11830] (ab170939)

© 2021 Astana Biomed Group LLP. All rights reserved.