Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PDE4B antibody [EPR11830] (ab170939)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11830] to PDE4B
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-PDE4B antibody [EPR11830]
See all PDE4B primary antibodies
Description

Rabbit monoclonal [EPR11830] to PDE4B
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment within Human PDE4B aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: Q07343
Positive control
- WB: HeLa, A549, Human fetal brain and Human glioma lysates; Mouse brain tissue lysate. Flow Cyt: A549 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11830
Isotype

IgG
Research areas

Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)

All lanes : Anti-PDE4B antibody [EPR11830] (ab170939) at 1/500 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PDE4B knockout HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Mouse brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 83 kDa
Observed band size: 83 kDa

Lanes 1-4: Merged signal (red and green). Green - ab170939 observed at 83 kDa. Red - loading control ab8245 observed at 36 kDa.

ab170939 Anti-PDE4B antibody [EPR11830] was shown to specifically react with PDE4B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265259 (knockout cell lysate ab257576) was used. Wild-type and PDE4B knockout samples were subjected to SDS-PAGE. ab170939 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-PDE4B antibody [EPR11830] (ab170939)

All lanes : Anti-PDE4B antibody [EPR11830] (ab170939) at 1/1000 dilution

Lane 1 : Human fetal brain lysates
Lane 2 : Human glioma lysates
Lane 3 : A549 lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 83 kDa
Flow Cytometry - Anti-PDE4B antibody [EPR11830] (ab170939)

Flow cytometric analysis of permeabilized A549 cells labeling PDE4B using ab170939 (red) at 1/10 dilution, or a rabbit IgG (negative) (green).
Anti-PDE4B antibody [EPR11830] (ab170939)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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