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Anti-Wnt2b antibody [EPR13386] (ab178418)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13386] to Wnt2b
  • Suitable for: ICC/IF, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Wnt2b antibody [EPR13386]
    See all Wnt2b primary antibodies

  • Description

    Rabbit monoclonal [EPR13386] to Wnt2b

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Wnt2b aa 250-350 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q93097

  • Positive control

    • WB: Human fetal brain and heart lysates; HepG2 and Jurkat cells and cell lysates. ICC/IF: HT-29 and HepG2 cells. Flow Cyt: Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Wnt2b antibody [EPR13386] (ab178418)
    Western blot - Anti-Wnt2b antibody [EPR13386] (ab178418)
    All lanes : Anti-Wnt2b antibody [EPR13386] (ab178418) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 44 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Wnt2b antibody [EPR13386] (ab178418)
    Immunocytochemistry/ Immunofluorescence - Anti-Wnt2b antibody [EPR13386] (ab178418)

    ab178418 staining Wnt2b in HT-29 (human colorectal adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control: PBS only.

  • Immunocytochemistry/ Immunofluorescence - Anti-Wnt2b antibody [EPR13386] (ab178418)
    Immunocytochemistry/ Immunofluorescence - Anti-Wnt2b antibody [EPR13386] (ab178418)

    Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Wnt2b with ab178418 at 1/50 dilution (green). DAPI nuclear staining (blue).

  • Flow Cytometry - Anti-Wnt2b antibody [EPR13386] (ab178418)
    Flow Cytometry - Anti-Wnt2b antibody [EPR13386] (ab178418)

    Flow Cytometric analysis of permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling Wnt2b with ab178418 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).

  • Anti-Wnt2b antibody [EPR13386] (ab178418)
    Anti-Wnt2b antibody [EPR13386] (ab178418)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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