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Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell pellet (ab279187)

Overview

  • Product name

    Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell pellet
    See all NPR-A kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.

  • Sequence similarities

    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 1 guanylate cyclase domain.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylation of the protein kinase-like domain is required for full activation by ANP.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • ANP-A
    • ANPa
    • ANPR-A
    • ANPRA
    • ANPRA_HUMAN
    • Atrial natriuretic peptide A type receptor
    • Atrial natriuretic peptide receptor 1
    • Atrial natriuretic peptide receptor A
    • Atrial natriuretic peptide receptor type A
    • Atrionatriuretic peptide receptor A
    • GC A
    • GC-A
    • Guanylate cyclase
    • Guanylate cyclase A
    • GUC2A
    • GUCY2A
    • Natriuretic peptide A type receptor
    • Natriuretic Peptide Receptor A
    • Natriuretic peptide receptor A/guanylate cyclase A
    • NPR-A
    • NPR1
    • NPRA
    see all

Properties

Images

  • Sanger Sequencing - Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell pellet (ab279187)
    Sanger Sequencing - Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell pellet (ab279187)

    Allele-1: 5 bp deletion in exon 1

     

  • Sanger Sequencing - Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell pellet (ab279187)
    Sanger Sequencing - Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell pellet (ab279187)

    Allele-2: 1 bp insertion in exon 1

     

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