Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DAP1 antibody [E59] (ab32056)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E59] to DAP1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-DAP1 antibody [E59]
    See all DAP1 primary antibodies

  • Description

    Rabbit monoclonal [E59] to DAP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human DAP1 aa 50-150 (C terminal). The exact sequence is proprietary.

  • Positive control

    • NIH3T3 cell lysate, Hela cell lysate and human stomach carcinoma

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DAP1 antibody [E59] (ab32056)
    Immunocytochemistry/ Immunofluorescence - Anti-DAP1 antibody [E59] (ab32056)
    Immunofluorescent analysis of DAP1 expression in HeLa cells using 1/250 ab32056.
  • Western blot - Anti-DAP1 antibody [E59] (ab32056)
    Western blot - Anti-DAP1 antibody [E59] (ab32056)
    Anti-DAP1 antibody [E59] (ab32056) at 1/10000 dilution + NIH3T3 cell lysate

    Observed band size: 11 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP1 antibody [E59] (ab32056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP1 antibody [E59] (ab32056)

    ab32056, at a 1/100 dilution, staining human stomach carcinoma by immunohistochemistry, Paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-DAP1 antibody [E59] (ab32056)
    Anti-DAP1 antibody [E59] (ab32056)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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