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Anti-Wnt2b antibody (ab92998)

Price and availability

268 032 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Wnt2b antibody (ab92998)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Wnt2b
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Wnt2b antibody
    See all Wnt2b primary antibodies
  • Description

    Rabbit polyclonal to Wnt2b
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Wnt2b aa 250-350 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab104425)

  • Positive control

    • This antibody gave a positive signal in Human heart tissue lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Secreted
    • Cancer
    • Signal transduction
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • PTC & Wnt pathway

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Wnt2b protein (ab132538)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Cow
Dog
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.
  • Tissue specificity

    Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines.
  • Sequence similarities

    Belongs to the Wnt family.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession Q93097 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 445420 Cow
    • Entrez Gene: 7482 Human
    • Entrez Gene: 22414 Mouse
    • Entrez Gene: 116466 Rat
    • Omim: 601968 Human
    • SwissProt: Q93097 Human
    • SwissProt: O70283 Mouse
    • Unigene: 258575 Human
    • Unigene: 10740 Mouse
    • Unigene: 17219 Rat
    see all
  • Alternative names

    • Protein Wnt-13 antibody
    • Protein Wnt-2b antibody
    • Wingless type MMTV integration site family, member 13 antibody
    • Wingless type MMTV integration site family, member 2B antibody
    • WNT13 antibody
    • Wnt2b antibody
    • WNT2B_HUMAN antibody
    • XWNT2 antibody
    • XWNT2, Xenopus, homolog of antibody
    see all

Images

  • Western blot - Anti-Wnt2b antibody (ab92998)
    Western blot - Anti-Wnt2b antibody (ab92998)
    Anti-Wnt2b antibody (ab92998) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa
    Additional bands at: 40 kDa, 40 kDa, 49 kDa, 49 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab92998? Please let us know so that we can cite the reference in this datasheet.

    ab92998 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-Wnt2b antibody (ab92998)
      Western blot - Anti-Wnt2b antibody (ab92998)
      Anti-Wnt2b antibody (ab92998) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 43 kDa
      Observed band size: 43 kDa
      Additional bands at: 40 kDa, 40 kDa, 49 kDa, 49 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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