Anti-WDHD1 antibody (ab224221)
Key features and details
- Rabbit polyclonal to WDHD1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-WDHD1 antibody
See all WDHD1 primary antibodies -
Description
Rabbit polyclonal to WDHD1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human WDHD1 aa 200-350.
Database link: O75717 -
Positive control
- WB: NIH/3T3, PC-12, RT4, U-251 MG sp and A431 cell lysates. ICC/IF: U-251 MG cells. IHC-P: Human tonsil tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-WDHD1 antibody (ab224221)
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for WDHD1 (green) using ab224221 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDHD1 antibody (ab224221)Paraffin-embedded human tonsil tissue stained for WDHD1 using ab224221 at 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-WDHD1 antibody (ab224221)All lanes : Anti-WDHD1 antibody (ab224221) at 1/250 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 126 kDa
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Western blot - Anti-WDHD1 antibody (ab224221)All lanes : Anti-WDHD1 antibody (ab224221) at 1/250 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 126 kDa
