Anti-ASC1 antibody (ab70627)
Key features and details
- Rabbit polyclonal to ASC1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ASC1 antibody
See all ASC1 primary antibodies -
Description
Rabbit polyclonal to ASC1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to amino acids 225-275 of Human ASC1 (NP_057297.2)
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Positive control
- HeLa whole cell lysate (ab150035) 293T whole cell lysate NIH3T3 whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ASC1 antibody (ab70627)All lanes : Anti-ASC1 antibody (ab70627) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-ASC1 antibody (ab70627)Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70627 at 3 µg/ml (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was used for Western blotting, analysed using ab70627 at 1µg/ml and detected by chemiluminescence for 10 seconds.
