Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VPS26 antibody [EPR13456] - BSA and Azide free (ab250480)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13456] to VPS26 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-VPS26 antibody [EPR13456] - BSA and Azide free
    See all VPS26 primary antibodies

  • Description

    Rabbit monoclonal [EPR13456] to VPS26 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WBmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250480 is the carrier-free version of ab181352. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250480 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-VPS26 antibody [EPR13456] - BSA and Azide free (ab250480)
    Western blot - Anti-VPS26 antibody [EPR13456] - BSA and Azide free (ab250480)

    This data was developed using ab181352, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: VPS26 knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: HepG2 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab181352 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab181352 was shown to specifically react with VPS26 when VPS26 knockout samples were used. Wild-type and VPS26 knockout samples were subjected to SDS-PAGE. ab181352 and ab18058 (loading control to Vinculin) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ( ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-VPS26 antibody [EPR13456] - BSA and Azide free (ab250480)
    Immunocytochemistry - Anti-VPS26 antibody [EPR13456] - BSA and Azide free (ab250480)
    This data was developed using ab181352, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling VPS26 with ab181352 at 1/100 dilution (red). DAPI nuclear staining (blue).
  • Western blot - Anti-VPS26 antibody [EPR13456] - BSA and Azide free (ab250480)
    Western blot - Anti-VPS26 antibody [EPR13456] - BSA and Azide free (ab250480)
    All lanes : Anti-VPS26 antibody [EPR13456] (ab181352) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 38 kDa



    This data was developed using ab181352, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-VPS26 antibody [EPR13456] - BSA and Azide free (ab250480)
    Immunoprecipitation - Anti-VPS26 antibody [EPR13456] - BSA and Azide free (ab250480)
    This data was developed using ab181352, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from HeLa cell lysate pulling down VPS26 using ab181352 at 1/10 dilution.
  • Anti-VPS26 antibody [EPR13456] - BSA and Azide free (ab250480)
    Anti-VPS26 antibody [EPR13456] - BSA and Azide free (ab250480)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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