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Anti-Vimentin (phospho S72) antibody [EP1070Y] - BSA and Azide free (ab232451)

Key features and details

  • Rabbit monoclonal [EP1070Y] to Vimentin (phospho S72) - BSA and Azide free
  • Suitable for: Dot blot, WB, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Vimentin (phospho S72) antibody [EP1070Y] - BSA and Azide free
    See all Vimentin primary antibodies

  • Description

    Rabbit monoclonal [EP1070Y] to Vimentin (phospho S72) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: C6 (starved for 24 hours then treated with 100 ng Calyculin A for 60 minutes) whole cell lysate.

  • General notes

    Ab232451 is the carrier-free version of ab52944. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232451 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Vimentin (phospho S72) antibody [EP1070Y] - BSA and Azide free (ab232451)
    Western blot - Anti-Vimentin (phospho S72) antibody [EP1070Y] - BSA and Azide free (ab232451)
    All lanes : Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944) at 1/10000 dilution

    Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
    Lane 2 : C6 (starved for 24 hours then treated with 100 ng Calyculin A for 60 minutes) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 54 kDa



    Blocking and diluting buffer : 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52944).

  • Immunoprecipitation - Anti-Vimentin (phospho S72) antibody [EP1070Y] - BSA and Azide free (ab232451)
    Immunoprecipitation - Anti-Vimentin (phospho S72) antibody [EP1070Y] - BSA and Azide free (ab232451)

    ab52944 (purified) at 1:20 dilution (2µg) immunoprecipitating Vimentin in HeLa starved 24 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) starved 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysate 10ug
    Lane 2 (+): ab52944 & HeLa starved 24 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52944 in HeLa starved 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52944).

  • Dot Blot - Anti-Vimentin (phospho S72) antibody [EP1070Y] - BSA and Azide free (ab232451)
    Dot Blot - Anti-Vimentin (phospho S72) antibody [EP1070Y] - BSA and Azide free (ab232451)

    Primary antibody dilution: 1/1000
    Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated (1/2500 dilution)
    Blocking & dilutiing buffer: 5% NFDM/TBST

    Lane 1 sample: Vimentin (pS72) phospho peptide
    Lane 2 sample: Vimentin non-phospho peptide

    Exposure time: 3 minutes

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52944).

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