Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-VAMP2 antibody [EPR12790] (ab181869)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12790] to VAMP2
Suitable for: WB, ICC/IF, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-VAMP2 antibody [EPR12790]
See all VAMP2 primary antibodies
Description

Rabbit monoclonal [EPR12790] to VAMP2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal brain and Human cerebellum lysates ICC/IF: SH-SY5Y cells. Flow cyt: Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12790
Isotype

IgG
Research areas

Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869)

Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/10000 dilution (purified) + Rat heart lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 13 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (ab181869)

ab181869 staining VAMP2 in U87-MG (human glioblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Negative control 1:PBS only.
Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (ab181869)

Immunofluorescent analysis of U87-MG cells (4% paraformaldehyde-fixed) labeling VAMP2 with ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution and counterstained with Dapi.
Flow Cytometry - Anti-VAMP2 antibody [EPR12790] (ab181869)

Flow cytometry analysis of Jurkat cells fixed with 2% paraformaldehyde labeling VAMP2 with unpurified ab181869 at 1/150 dilution followed by Goat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.
Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869)

Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/50000 dilution (purified) + Mouse brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 13 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869)

Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/50000 dilution (purified) + Human cerebellum lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 13 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869)

All lanes : Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/50000 dilution (unpurified)

Lane 1 : Human fetal brain lysate
Lane 2 : Human cerebellum lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 13 kDa
Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] (ab181869)

ab181869 (purified) at 1/150 immunoprecipitating VAMP2 in Human cerebellum whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.
Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] (ab181869)

Western blot analysis of immunoprecipitation pellet from Human cerebellum lysate immunoprecipitated using ab181869 at 1/70 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Flow Cytometry - Anti-VAMP2 antibody [EPR12790] (ab181869)

Flow Cytometry analysis of SH-SY5Y cells labelling VAMP2 with purified ab181869 at 1/100 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (ab181869)

Immunocytochemistry/Immunofluorescence analysis of U87-MG (human glioblastoma) cells labelling VAMP2 with purified ab181869 at 1/250. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (ab181869)

Immunofluorescent analysis of SH-SYSY cells (human cell line) (4% paraformaldehyde-fixed) labeling VAMP2 with unpurified ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor^®488) at 1/200 dilution and counterstained with DAPI.
Anti-VAMP2 antibody [EPR12790] (ab181869)

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