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Human SNAP29 knockout HeLa cell pellet (ab279085)

Product name

Human SNAP29 knockout HeLa cell pellet
See all SNAP29 kits
Product overview

Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.
Parental Cell Line

HeLa
Organism

Human
Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 5 bp deletion in exon1.
Passage number
Knockout validation

Sanger Sequencing, Western Blot (WB)
Notes

Pellet size: 5 million cells/vial.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Tested applications

Suitable for: WBmore details

Storage instructions

Store at -80°C. Please refer to protocols.
Components 1 kit
Human SNAP29 knockout HeLa cell pellet 1 vial
Human wild-type HeLa cell pellet 1 vial
Research areas
Cell type

epithelial
Disease

Adenocarcinoma
Gender

Female
STR Analysis

Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Components	1 kit
Human SNAP29 knockout HeLa cell pellet	1 vial
Human wild-type HeLa cell pellet	1 vial

Function

Involved in multiple membrane trafficking steps.
Tissue specificity

Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.
Involvement in disease

Defects in SNAP29 are the cause of CEDNIK syndrome (CEDNIK) [MIM:609528]. CEDNIK is a neurocutaneous syndrome characterized by cerebral dysgenesis, neuropathy, ichthyosis and palmoplantar keratoderma.
Sequence similarities

Belongs to the SNAP-25 family.
Contains 1 t-SNARE coiled-coil homology domain.
Cellular localization

Cytoplasm. Membrane. Cell junction > synapse > synaptosome. Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic.
Target information above from: UniProt accession O95721 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Alternative names
- CEDNIK
- FLJ21051
- SNAP 29
- SNAP-29
- SNAP29
- SNP29_HUMAN
- Soluble 29 kDa NSF attachment protein
- Synaptosomal associated protein 29
- Synaptosomal associated protein 29kDa
- Synaptosomal-associated protein 29
- Vesicle membrane fusion protein SNAP 29
- Vesicle membrane fusion protein SNAP29
- Vesicle-membrane fusion protein SNAP-29
see all

Storage instructions

Store at -80°C. Please refer to protocols.
Components 1 kit
Human SNAP29 knockout HeLa cell pellet 1 vial
Human wild-type HeLa cell pellet 1 vial
Research areas
Cell type

epithelial
Disease

Adenocarcinoma
Gender

Female
STR Analysis

Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Components	1 kit
Human SNAP29 knockout HeLa cell pellet	1 vial
Human wild-type HeLa cell pellet	1 vial

Western blot - Human SNAP29 knockout HeLa cell pellet (ab279085)

Lane 1:Wild-type HeLa cell lysate (20 ug)
Lane 2:SNAP29 knockout HeLa cell lysate (20 ug)
Lane 3:HepG2 cell lysate (20 ug)

ab138500 was shown to specifically react with SNAP29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265289 (knockout cell lysate ab257693) was used. Wild-type and SNAP29 knockout samples were subjected to SDS-PAGE. ab138500 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Human SNAP29 knockout HeLa cell pellet (ab279085)

Lane 1:Wild-type HeLa cell lysate (20 ug)
Lane 2:SNAP29 knockout HeLa cell lysate (20 ug)
Lane 3:HepG2 cell lysate (20 ug)

ab181151 was shown to specifically react with SNAP29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265289 (knockout cell lysate ab257693) was used. Wild-type and SNAP29 knockout samples were subjected to SDS-PAGE. ab181151 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Sanger Sequencing - Human SNAP29 knockout HeLa cell pellet (ab279085)

Allele-1: 5 bp deletion in exon1
Sanger Sequencing - Human SNAP29 knockout HeLa cell pellet (ab279085)

Allele-2: 1 bp insertion in exon1