Anti-V5 tag antibody (ab9113)
Key features and details
- Chicken polyclonal to V5 tag
- Suitable for: ELISA, WB, ICC/IF, ICC
- Reacts with: Species independent
- Isotype: unknown
Overview
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Product name
Anti-V5 tag antibody
See all V5 tag primary antibodies -
Description
Chicken polyclonal to V5 tag -
Host species
Chicken -
Specificity
Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:100,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP conjugated goat anti chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run. -
Tested applications
Suitable for: ELISA, WB, ICC/IF, ICCmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
GKPIPNPLLGLDST (V5 epitope) conjugated with KLH.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
IgY fraction -
Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid support. Antibody concentration was determined by extinction coefficient : O.D. 1.32 at 280nM equals 1.0 mg of IgY (IgG). -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody (ab9113) This image is courtesy of an anonymous Abreviewab9113 staining human fibrosarcoma cells (HT1080) by ICC/IF. Cells were transfected with V5- tagged transmembrane protein and PFA fixed without undergoing permeabilization and blocking steps. The primary antibody was used at 10µg/ml and incubated with the sample for 1 hour at 20°C. An Alexa Fluor® 546 conjugated donkey polyclonal anti-chicken (H+L) antibody, diluted 1/500, was used as the secondary.
