Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-USP5 antibody [EPR10454] - BSA and Azide free (ab249070)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10454] to USP5 - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-USP5 antibody [EPR10454] - BSA and Azide free
    See all USP5 primary antibodies

  • Description

    Rabbit monoclonal [EPR10454] to USP5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7 and A549 whole cell lysates. Human, mouse, and rat brain tissue lysates. Mouse and rat heart and kidney tissue lysates; ICC: MCF7 and A549 cells; Flow Cyt: MCF7 cells.

  • General notes

    Ab249070 is the carrier-free version of ab154170. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249070 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-USP5 antibody [EPR10454] - BSA and Azide free (ab249070)
    Western blot - Anti-USP5 antibody [EPR10454] - BSA and Azide free (ab249070)
    All lanes : Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution (purified)

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 3 : Human brain lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Mouse heart lysate
    Lane 7 : Rat heart lysate
    Lane 8 : Mouse kidney lysate
    Lane 9 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 96 kDa
    Observed band size: 96 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154170).

  • Immunocytochemistry - Anti-USP5 antibody [EPR10454] - BSA and Azide free (ab249070)
    Immunocytochemistry - Anti-USP5 antibody [EPR10454] - BSA and Azide free (ab249070)

    Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling USP5 with purified ab154170 at 1/50 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS,BSA,glycerol,and sodium azide (ab154170).

  • Flow Cytometry - Anti-USP5 antibody [EPR10454] - BSA and Azide free (ab249070)
    Flow Cytometry - Anti-USP5 antibody [EPR10454] - BSA and Azide free (ab249070)

    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling USP5 with purified ab154170 at 1/50 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154170).

  • Anti-USP5 antibody [EPR10454] - BSA and Azide free (ab249070)
    Anti-USP5 antibody [EPR10454] - BSA and Azide free (ab249070)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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