Anti-UBPY/USP8 antibody (ab228572)
Key features and details
- Rabbit polyclonal to UBPY/USP8
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UBPY/USP8 antibody
See all UBPY/USP8 primary antibodies -
Description
Rabbit polyclonal to UBPY/USP8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey -
Immunogen
Recombinant fragment within Human UBPY/USP8 (internal sequence). The exact sequence is proprietary.
Database link: P40818 -
Positive control
- ICC/IF: A549 cells. IHC-P: Human colon carcinoma tissue. WB: NCI-H1299 and HCT-116 whole cell lysate. IP: HCT-116 whole cell extracts.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-UBPY/USP8 antibody (ab228572) at 1/5000 dilution
Lane 1 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HCT-116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 128 kDa5% SDS-PAGE
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A549 (human lung carcinoma cell line) cells stained for UBPY/USP8 (green) using ab228572 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).
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Paraffin-embedded human colon carcinoma tissue stained for UBPY/USP8 with ab228572 at 1/500 dilution in immunohistochemical analysis.
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UBPY/USP8 was immunoprecipitated from HCT-116 (human colorectal carcinoma cell line) whole cell extract using 5 µg of ab228572. Western blot was performed from the immunoprecipitate using ab228572.
Lane 1: HCT-116 whole cell extract (Input).
Lane 2: Control IgG IP in HCT-116 whole cell extract.
Lane 3: ab228572 IP in HCT-116 whole cell extract.