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Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)

Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15591] to UQCRB - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-UQCRB antibody [EPR15591] - BSA and Azide free
    See all UQCRB primary antibodies
  • Description

    Rabbit monoclonal [EPR15591] to UQCRB - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251022 is the carrier-free version of ab190360. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251022 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR15591
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cytochromes
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Cytochromes

Images

  • Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
    Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
    All lanes : Anti-UQCRB antibody [EPR15591] (ab190360) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa



    This data was developed using ab190360, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
    Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
    Anti-UQCRB antibody [EPR15591] (ab190360) at 1/5000 dilution + 293 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa



    This data was developed using ab190360, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
    Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
    All lanes : Anti-UQCRB antibody [EPR15591] (ab190360) at 1/1000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa



    This data was developed using ab190360, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
    This data was developed using ab190360, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adenocarcinoma of the colon labeling UQCRB with ab190360 at 1/100 and HRP Polymer for Rabbit IgG. Counterstained with hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
    Immunocytochemistry - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
    This data was developed using ab190360, the same antibody clone in a different buffer formulation.Immunocytochemical analysis of HeLa cells fixed in 4% paraformaldehyde labeling UQCRB with ab190360 at 1/250 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Counterstained with DAPI. Negative controls also shown.
  • Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
    Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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