Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-NDUFA1 antibody [EPR12083] (ab176563)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12083] to NDUFA1
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-NDUFA1 antibody [EPR12083]
See all NDUFA1 primary antibodies
Description

Rabbit monoclonal [EPR12083] to NDUFA1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFA1 aa 1 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O15239
Positive control
- A673 cell lysate; Human skeletal muscle, Human fetal kidney and fetal heart lysates; Human heart and skeletal muscle tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12083
Isotype

IgG
Research areas

Western blot - Anti-NDUFA1 antibody [EPR12083] (ab176563)

All lanes : Anti-NDUFA1 antibody [EPR12083] (ab176563) at 1/1000 dilution

Lane 1 : A673 cell lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human skeletal muscle lysate
Lane 4 : Human fetal heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 8 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] (ab176563)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human heart tissue labeling NDUFA1 with ab176563 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA1 antibody [EPR12083] (ab176563)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human skeletal muscle tissue labeling NDUFA1 with ab176563 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-NDUFA1 antibody [EPR12083] (ab176563)

Western blot analysis on immunoprecipitation pellet from Human fetal heart lysate labeling NDUFA1 with ab176563 at 1/10 dilution.
Anti-NDUFA1 antibody [EPR12083] (ab176563)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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