Antibodies, Proteins, Kits and Reagents for Life Science

247 259 ₸ Product size

100 µl 247 259 ₸

Order now and get it on Thursday March 04, 2021

Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19006] to UCP3 + UCP1
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-UCP3 + UCP1 antibody [EPR19006]
See all UCP3+UCP1 primary antibodies
Description

Rabbit monoclonal [EPR19006] to UCP3 + UCP1
Host species

Rabbit

Tested Applications & Species

Application	Species
IP	Mouse
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse UCP1 full length recombinant protein; Mouse UCP3 full length recombinant protein; Mouse muscle and brown adipose lysates; Rat brown adipose lysate. IP: Mouse skeletal muscle whole cell lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19006
Isotype

IgG
Research areas

Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

All lanes : Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution

Lane 1 : Mouse UCP1 full length recombinant protein
Lane 2 : Mouse UCP2 full length recombinant protein

Lysates/proteins at 0.02 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 34, 33 kDa
Observed band size: 34 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Mouse UCP1 full length recombinant protein contains aa1-307 with a His-Tag®. Mouse UCP2 full length recombinant protein contains aa1-309 with a His-Tag®. These two full length recombinant proteins were made in-house
Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution + Mouse UCP3 full length recombinant protein at 0.02 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 34, 33 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.

Mouse UCP3 full length recombinant protein contains aa1-308 with a GST-Tag. This full length recombinant protein was made in-house.
Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

All lanes : Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution

Lane 1 : Mouse muscle lysate
Lane 2 : Mouse lung lysate
Lane 3 : C2C12 (Mouse myoblast cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 34, 33 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID: 9080264)
Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

All lanes : Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution

Lane 1 : Mouse brown adipose lysate
Lane 2 : Rat brown adipose lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 34, 33 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 1 minute; Lane 2: 15 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID: 9080264).
Immunoprecipitation - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

UCP3 + UCP1 were immunoprecipitated from 1mg of Mouse skeletal muscle whole cell lysate with ab193470 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab193470 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Mouse skeletal muscle whole cell lysate, 10µg (Input).

Lane 2: ab193470 IP in Mouse skeletal muscle whole cell lysate.

Lane 3: Rabbit IgG, monoclonal - Isotype Control (ab172730) instead of ab193470 in Mouse skeletal muscle whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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