Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19006] to UCP3 + UCP1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UCP3 + UCP1 antibody [EPR19006]
    See all UCP3+UCP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19006] to UCP3 + UCP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse UCP1 full length recombinant protein; Mouse UCP3 full length recombinant protein; Mouse muscle and brown adipose lysates; Rat brown adipose lysate. IP: Mouse skeletal muscle whole cell lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)
    Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)
    All lanes : Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution

    Lane 1 : Mouse UCP1 full length recombinant protein
    Lane 2 : Mouse UCP2 full length recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34, 33 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Mouse UCP1 full length recombinant protein contains aa1-307 with a His-Tag®. Mouse UCP2 full length recombinant protein contains aa1-309 with a His-Tag®.  These two full length recombinant proteins were made in-house

  • Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)
    Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)
    Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution + Mouse UCP3 full length recombinant protein at 0.02 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34, 33 kDa
    Observed band size: 34 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Mouse UCP3 full length recombinant protein contains aa1-308 with a GST-Tag. This full length recombinant protein was made in-house.

  • Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)
    Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)
    All lanes : Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution

    Lane 1 : Mouse muscle lysate
    Lane 2 : Mouse lung lysate
    Lane 3 : C2C12 (Mouse myoblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34, 33 kDa
    Observed band size: 34 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 9080264)

  • Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)
    Western blot - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)
    All lanes : Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470) at 1/1000 dilution

    Lane 1 : Mouse brown adipose lysate
    Lane 2 : Rat brown adipose lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34, 33 kDa
    Observed band size: 34 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time:  Lane 1: 1 minute; Lane 2: 15 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 9080264).

  • Immunoprecipitation - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)
    Immunoprecipitation - Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

    UCP3 + UCP1 were immunoprecipitated from 1mg of Mouse skeletal muscle whole cell lysate with ab193470 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab193470 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse skeletal muscle whole cell lysate,  10µg (Input).

    Lane 2: ab193470 IP in Mouse skeletal muscle whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal - Isotype Control (ab172730) instead of ab193470 in Mouse skeletal muscle whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)
    Anti-UCP3 + UCP1 antibody [EPR19006] (ab193470)

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