Anti-UBE2I / UBC9 antibody (ab233224)
Key features and details
- Rabbit polyclonal to UBE2I / UBC9
- Suitable for: WB, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-UBE2I / UBC9 antibody
See all UBE2I / UBC9 primary antibodies -
Description
Rabbit polyclonal to UBE2I / UBC9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Chicken, Zebrafish
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Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human UBE2I/ UBC9 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P63279 -
Positive control
- WB: Recombinant human UBE2I / UBC9 protein; Human lung lysate; HeLa cell lysate; Pig spleen and uterus lysates. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-UBE2I / UBC9 antibody (ab233224)Anti-UBE2I / UBC9 antibody (ab233224) at 2 µg + Recombinant human UBE2I / UBC9 protein
Predicted band size: 18 kDa
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Western blot - Anti-UBE2I / UBC9 antibody (ab233224)All lanes : Anti-UBE2I / UBC9 antibody (ab233224) at 2 µg/ml
Lane 1 : Human lung lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Pig spleen lysate
Lane 4 : Pig uterus lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 18 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-UBE2I / UBC9 antibody (ab233224)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for UBE2I / UBC9 (green) using ab233224 at 20 μg/ml in ICC/IF. FITC staining.
