Anti-UACA/Nucling antibody (ab99323)
Key features and details
- Rabbit polyclonal to UACA/Nucling
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UACA/Nucling antibody
See all UACA/Nucling primary antibodies -
Description
Rabbit polyclonal to UACA/Nucling -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human UACA/Nucling aa 1050-1150.
Database link: NP_060473.2 -
Positive control
- HeLa and 293T whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-UACA/Nucling antibody (ab99323)All lanes : Anti-UACA/Nucling antibody (ab99323) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 162 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-UACA/Nucling antibody (ab99323)Detection of UACA/Nucling in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded), using ab99323 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
