Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
![Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)](https://www.abcam.com/ps/products/188/ab188328/Images/ab188328-410165-anti-txnl1-antibody-epr16061b-n-terminal-western-blot-human-knockout-lysate.jpg "Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16061(B)] to TXNL1 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-TXNL1 antibody [EPR16061(B)] - N-terminal
See all TXNL1 primary antibodies -
Description
Rabbit monoclonal [EPR16061(B)] to TXNL1 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB RatHuman
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Caco 2, K562, 293, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates. IHC-P: Human thyroid papillary adenocarcinoma tissue. ICC: HCT-116 cells. Flow Cyt: 293 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16061(B) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : TXNL1 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDaLanes 1-4: Merged signal (red and green). Green - ab188328 observed at 32 kDa. Red - loading control ab7291 observed at 50 kDa.
ab188328 Anti-TXNL1 antibody [EPR16061(B)] - N-terminal was shown to specifically react with TXNL1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266413 (knockout cell lysate ab258258) was used. Wild-type and TXNL1 knockout samples were subjected to SDS-PAGE. ab188328 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)](https://www.abcam.com/ps/products/188/ab188328/Images/ab188328-224257-anti-txnl1-antibody-epr16061b-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)Immunohistochemical analysis of paraffin-embedded Human thyroid papillary adenocarcinoma tissue labeling TXNL1 with ab188328 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)](https://www.abcam.com/ps/products/188/ab188328/Images/ab188328-224258-anti-txnl1-antibody-epr16061b-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)Immunofluorescent analysis of HCT-116 cells (paraformaldehyde-fixed, 4%) labeling TXNL1 with ab188328 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
-
![Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)](https://www.abcam.com/ps/products/188/ab188328/Images/ab188328-224255-anti-txnl1-antibody-epr16061b-n-terminal-western-blot.jpg)
Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/10000 dilution + 293 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 32 kDa
-
![Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)](https://www.abcam.com/ps/products/188/ab188328/Images/ab188328-224256-anti-txnl1-antibody-epr16061b-n-terminal-western-blot.jpg)
Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/10000 dilution
Lane 1 : Caco 2 cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 32 kDa
-
![Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)](https://www.abcam.com/ps/products/188/ab188328/Images/ab188328-224254-anti-txnl1-antibody-epr16061b-n-terminal-western-blot.jpg)
Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 32 kDa
-
![Flow Cytometry - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)](https://www.abcam.com/ps/products/188/ab188328/Images/ab188328-224260-anti-txnl1-antibody-epr16061b-n-terminal-flow-cytometry.jpg)
Flow Cytometry - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)Flow Cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling TXNL1 with ab188328 at 1/80 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
-
![Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)](https://www.abcam.com/ps/products/188/ab188328/Images/ab188328-8-benefits-of-recombinant-antibodies.png)
Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
