Antibodies, Proteins, Kits and Reagents for Life Science

268 032 ₸ Product size

100 µl 268 032 ₸

Order now and get it on Tuesday March 09, 2021

Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16061(B)] to TXNL1 - N-terminal
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-TXNL1 antibody [EPR16061(B)] - N-terminal
See all TXNL1 primary antibodies
Description

Rabbit monoclonal [EPR16061(B)] to TXNL1 - N-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Caco 2, K562, 293, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates. IHC-P: Human thyroid papillary adenocarcinoma tissue. ICC: HCT-116 cells. Flow Cyt: 293 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16061(B)
Isotype

IgG
Research areas

Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : TXNL1 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Caco-2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa

Lanes 1-4: Merged signal (red and green). Green - ab188328 observed at 32 kDa. Red - loading control ab7291 observed at 50 kDa.

ab188328 Anti-TXNL1 antibody [EPR16061(B)] - N-terminal was shown to specifically react with TXNL1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266413 (knockout cell lysate ab258258) was used. Wild-type and TXNL1 knockout samples were subjected to SDS-PAGE. ab188328 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

Immunohistochemical analysis of paraffin-embedded Human thyroid papillary adenocarcinoma tissue labeling TXNL1 with ab188328 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

Immunofluorescent analysis of HCT-116 cells (paraformaldehyde-fixed, 4%) labeling TXNL1 with ab188328 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/10000 dilution + 293 cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa
Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/10000 dilution

Lane 1 : Caco 2 cell lysate
Lane 2 : K562 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa
Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa
Flow Cytometry - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

Flow Cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling TXNL1 with ab188328 at 1/80 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

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