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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules Tubulin

Anti-TUBA1C antibody (ab222849)

Price and availability

264 681 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-TUBA1C antibody (ab222849)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to TUBA1C
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-TUBA1C antibody
  • Description

    Rabbit polyclonal to TUBA1C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chinese hamster
  • Immunogen

    Recombinant fragment corresponding to Human TUBA1C aa 1-200.
    Database link: Q9BQE3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: K562, PC-3, and U-251 MG whole cell lysates and mouse brain lysate. IHC-P: Human prostate cancer tissue. IP: K562 whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin

Images

  • Western blot - Anti-TUBA1C antibody (ab222849)
    Western blot - Anti-TUBA1C antibody (ab222849)
    All lanes : Anti-TUBA1C antibody (ab222849) at 1/500 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 3 : U-251 MG (human brain glioma cell line) whole cell lysate
    Lane 4 : Mouse brain tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA1C antibody (ab222849)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA1C antibody (ab222849)

    Paraffin-embedded human prostate cancer tissue stained for TUBA1C with ab222849 at 1/100 dilution in immunohistochemical analysis.

  • Immunoprecipitation - Anti-TUBA1C antibody (ab222849)
    Immunoprecipitation - Anti-TUBA1C antibody (ab222849)

    TUBA1C was immunopreciptated from K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate.

    Lane 1: Rabbit monoclonal IgG (1 µg) instead of ab222849 in K562 whole cell lysate. 

    Lane 2: ab222849 (4 µg) + K562 whole cell lysate (500 µg)

    Lane 3: K562 whole cell lysate 20 µg (Input)

    For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/50000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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