Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] - BSA and Azide free (ab247596)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3424] to Tryptophanyl tRNA synthetase/WRS - BSA and Azide free
- Suitable for: IHC-P, ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] - BSA and Azide free
See all Tryptophanyl tRNA synthetase/WRS primary antibodies -
Description
Rabbit monoclonal [EPR3424] to Tryptophanyl tRNA synthetase/WRS - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247596 is the carrier-free version of ab92733 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247596 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3424 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733) at 1/10000 dilution
Lane 1 : JAR cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDaThis data was developed using ab92733, the same antibody clone in a different buffer formulation.
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This data was developed using ab92733, the same antibody clone in a different buffer formulation.Immunofluorescence staining of MCF7 cells with purified ab92733 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
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