Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23495-276] to CREBBP + KAT3B / p300 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR23495-276] to CREBBP + KAT3B / p300 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICCmore details
    Unsuitable for: ChIP or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, K-562, RAW264.7 and PC-12 whole cell lysates; His-tagged human KAT3B/p300 recombinant protein;His-tagged human CREBBP recombinant protein. IHC-P: Human kidney, colon, clear cell renal carcinoma and hepatocellular carcinoma tissue. ICC: HeLa cells. Flow cyt: HeLa cells.

  • General notes

    ab275389 is the carrier-free version of ab275379. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275389 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)

    This data was developed using ab275379, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human hepatocellular carcinoma (PMID: 32144235). The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunocytochemistry - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)
    Immunocytochemistry - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)

    This data was developed using ab275379, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling KAT3B/p300+CREBBP with ab275379 at 1/100 (4.67 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in HeLa cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000  dilution.

  • Flow Cytometry - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)
    Flow Cytometry - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)

    This data was developed using ab275379, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling KAT3B/p300+CREBBP with ab275379 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)
    Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)
    All lanes : Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379) at 1/1000 dilution

    Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 2 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), at 1/100000 dilution
    Lanes 2-3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Observed band size: 300 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab275379, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 8995708).

    Lysates were made freshly and used in WB immediately to minimize protein degradation.

    Exposure times: Lane 1: 48 seconds; Lane 2: 70 seconds; Lane 3: 59 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)

    This data was developed using ab275379, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human clear cell renal cell carcinoma tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human clear cell renal cell carcinoma (PMID: 28551630). The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)
    Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)
    All lanes : Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379) at 1/1000 dilution

    Lane 1 : His-tagged human KAT3B/p300 recombinant protein (aa 1287-1666) 20 ng
    Lane 2 : His-tagged human CREBBP recombinant protein, E. coli extract, 10 µl

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution


    This data was developed using ab275379, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This product can recognize both KAT3B/p300 and CREBBP.

    Exposure time: 3 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)

    This data was developed using ab275379, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human colon (PMID: 28586030). The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)
    Western blot - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)
    Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] (ab275379) at 1/1000 dilution + HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/5000 dilution

    Observed band size: 300 kDa why is the actual band size different from the predicted?



    This data was developed using ab275379, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 8995708).

    Lysate was made freshly and used in WB immediately to minimize protein degradation.

    Exposure time: 32 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)

    This data was developed using ab275379, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling KAT3B/p300+CREBBP with ab275379 at 1/5000 (0.093 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human kidney (PMID: 28551630). The section was incubated with ab275379 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)
    Anti-CREBBP + KAT3B / p300 antibody [EPR23495-276] - BSA and Azide free (ab275389)

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