Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸ 10 µl 103 862 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Trypsin antibody [EPR19498] (ab200997)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19498] to Trypsin
Suitable for: WB, IHC-P
Reacts with: Human, Recombinant fragment

Product name

Anti-Trypsin antibody [EPR19498]
See all Trypsin primary antibodies
Description

Rabbit monoclonal [EPR19498] to Trypsin
Host species

Rabbit
Specificity

This product reacts with human Trypsin 1, Trypsin 3 and Putative trypsin 6 but not with Trypsin 2. However, it might cross react with other family members and isoforms.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

Recombinant fragment

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human PRSS1 fragment recombinant; Human PRSS3 full length recombinant protein; Human PRSS3P2 full length recombinant protein; Human pancreas lysate. IHC-P: Human pancreas, cerebrum and pancreas cancer tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Application	Species
IHC-P	Human
WB	Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19498
Isotype

IgG
Research areas

Western blot - Anti-Trypsin antibody [EPR19498] (ab200997)

All lanes : Anti-Trypsin antibody [EPR19498] (ab200997) at 1/1000 dilution

Lane 1 : Human PRSS1 fragment recombinant protein
Lane 2 : Human PRSS2 full length recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 27, 33 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 1 second; Lane 2: 3 minutes.

Human PRSS1 fragment recombinant protein contains aa123-247 with a GST-Tag; Human PRSS2 full length recombinant protein contains aa1-247 with a GST-Tag. These two recombinant proteins were made in-house.
Western blot - Anti-Trypsin antibody [EPR19498] (ab200997)

All lanes : Anti-Trypsin antibody [EPR19498] (ab200997) at 1/1000 dilution

Lane 1 : Human PRSS3 full length recombinant protein
Lane 2 : Human PRSS3P2 full length recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 27, 33 kDa
Observed band size: 27 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Human PRSS3 full length recombinant protein contains aa1-304 with a His-Tag®. Human PRSS3P2 full length recombinant protein contains aa1-247 with a His-Tag®. These two recombinant proteins were made in-house.
Western blot - Anti-Trypsin antibody [EPR19498] (ab200997)

All lanes : Anti-Trypsin antibody [EPR19498] (ab200997) at 1/2000 dilution

Lane 1 : Human pancreas lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Human fetal kidney lysate
Lane 5 : Human fetal spleen lysate
Lane 6 : Human fetal lung lysate
Lane 7 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate
Lane 8 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 9 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 27, 33 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Trypsinogens I (PRSS1), Trypsinogens II (PRSS2), and mesotrypsinogen (PRSS3) are the major trypsinogen genes in the human pancreas. Trypsinogens I and II are the principal forms of trypsin that are secreted in pancreatic juice, while mesotrypsinogen is a minor trypsinogen in human pancreatic juice [PMID: 14726524, 3011602, 6698368, and 9099703]. This product recognizes PRSS1, and shows some cross-reactivity with PRSS3 and PRSS3P2 proteins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498] (ab200997)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Trypsin with ab200997 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on acinar cells of Human pancreas is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498] (ab200997)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Trypsin with ab200997 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human cerebrum is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498] (ab200997)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Trypsin with ab200997 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human colon. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498] (ab200997)

Immunohistochemical analysis of paraffin-embedded Human pancreas cancer tissue labeling Trypsin with ab200997 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human pancreas cancer is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Trypsin antibody [EPR19498] (ab200997)

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