Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Trypsin antibody [EPR19498-15] (ab211491)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19498-15] to Trypsin
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Trypsin antibody [EPR19498-15]
    See all Trypsin primary antibodies

  • Description

    Rabbit monoclonal [EPR19498-15] to Trypsin

  • Host species

    Rabbit

  • Specificity

    This product reacts with human Trypsin 1, Trypsin 2, Trypsin 3 and putative trypsin 6. It might cross react with other family members and isoforms due to high sequence homology.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human PRSS1 fragment recombinant protein; Human PRSS2 full length recombinant protein; Human PRSS3 full length recombinant protein; Human PRSS3P2 full length recombinant protein; Human pancreas lysate. IHC-P: Human pancreas tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/1000 dilution

    Lane 1 : Human PRSS1 fragment recombinant protein
    Lane 2 : Human PRSS2 full length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa
    Observed band size: 40,53 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human PRSS1 fragment recombinant protein contains aa123-247 with a GST-Tag; Human PRSS2 full length recombinant protein contains aa1-247 with a GST-Tag. These two recombinant proteins were made in-house.

  • Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/1000 dilution

    Lane 1 : Human PRSS3 full length recombinant protein
    Lane 2 : Human PRSS3P2 full length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa
    Observed band size: 28,38 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 5 seconds; Lane 2: 1 minute.

    Human PRSS3 full length recombinant protein contains aa1-304 with a His-Tag®. Human PRSS3P2 full length recombinant protein contains aa1-247 with a His-Tag®. These two recombinant proteins were made in-house.

  • Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/5000 dilution

    Lane 1 : Human pancreas lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal lung lysate
    Lane 5 : Human fetal spleen lysate
    Lane 6 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa


    Exposure time: 2 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Trypsinogens I (PRSS1), Trypsinogens II (PRSS2), and mesotrypsinogen (PRSS3) are the major trypsinogen genes in the human pancreas. Trypsinogens I and II are the principal forms of trypsin that are secreted in pancreatic juice, while mesotrypsinogen is a minor trypsinogen in human pancreatic juice [PMID: 14726524, 3011602, 6698368, and 9099703]. PRSS3P2 is putative trypsin-6. This product recognizes all four trypsins. Pancreas is the positive tissue, while other non-related tissues are chosen as negative controls.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Trypsin with ab211491 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human pancreas acinar cells is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Trypsin with ab211491 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: negative staining on Human colon.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Trypsin antibody [EPR19498-15] (ab211491)
    Anti-Trypsin antibody [EPR19498-15] (ab211491)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Trypsin antibody [EPR19498-15] (ab211491)

© 2021 Astana Biomed Group LLP. All rights reserved.