Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Thursday March 04, 2021

Anti-Trypsin antibody [EPR19498-15] (ab211491)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19498-15] to Trypsin
Suitable for: WB, IHC-P
Reacts with: Human, Recombinant fragment

Product name

Anti-Trypsin antibody [EPR19498-15]
See all Trypsin primary antibodies
Description

Rabbit monoclonal [EPR19498-15] to Trypsin
Host species

Rabbit
Specificity

This product reacts with human Trypsin 1, Trypsin 2, Trypsin 3 and putative trypsin 6. It might cross react with other family members and isoforms due to high sequence homology.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

Recombinant fragment

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human PRSS1 fragment recombinant protein; Human PRSS2 full length recombinant protein; Human PRSS3 full length recombinant protein; Human PRSS3P2 full length recombinant protein; Human pancreas lysate. IHC-P: Human pancreas tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19498-15
Isotype

IgG
Research areas

Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)

All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/1000 dilution

Lane 1 : Human PRSS1 fragment recombinant protein
Lane 2 : Human PRSS2 full length recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 27 kDa
Observed band size: 40,53 kDa
why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Human PRSS1 fragment recombinant protein contains aa123-247 with a GST-Tag; Human PRSS2 full length recombinant protein contains aa1-247 with a GST-Tag. These two recombinant proteins were made in-house.
Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)

All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/1000 dilution

Lane 1 : Human PRSS3 full length recombinant protein
Lane 2 : Human PRSS3P2 full length recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 27 kDa
Observed band size: 28,38 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 5 seconds; Lane 2: 1 minute.

Human PRSS3 full length recombinant protein contains aa1-304 with a His-Tag®. Human PRSS3P2 full length recombinant protein contains aa1-247 with a His-Tag®. These two recombinant proteins were made in-house.
Western blot - Anti-Trypsin antibody [EPR19498-15] (ab211491)

All lanes : Anti-Trypsin antibody [EPR19498-15] (ab211491) at 1/5000 dilution

Lane 1 : Human pancreas lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Human fetal lung lysate
Lane 5 : Human fetal spleen lysate
Lane 6 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Exposure time: 2 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Trypsinogens I (PRSS1), Trypsinogens II (PRSS2), and mesotrypsinogen (PRSS3) are the major trypsinogen genes in the human pancreas. Trypsinogens I and II are the principal forms of trypsin that are secreted in pancreatic juice, while mesotrypsinogen is a minor trypsinogen in human pancreatic juice [PMID: 14726524, 3011602, 6698368, and 9099703]. PRSS3P2 is putative trypsin-6. This product recognizes all four trypsins. Pancreas is the positive tissue, while other non-related tissues are chosen as negative controls.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Trypsin with ab211491 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human pancreas acinar cells is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-15] (ab211491)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Trypsin with ab211491 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control: negative staining on Human colon.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Trypsin antibody [EPR19498-15] (ab211491)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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