Anti-TRPM4 antibody [OTI10H5] (ab123936)
![Anti-TRPM4 antibody [OTI10H5] (ab123936)](https://www.abcam.com/ps/products/123/ab123936/Images/ab123936-137367-anti-trpm4-antibody-oti10h5-western-blot.jpg "Anti-TRPM4 antibody [OTI10H5] (ab123936)")
Key features and details
- Mouse monoclonal [OTI10H5] to TRPM4
- Suitable for: WB, IHC-P
- Reacts with: Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-TRPM4 antibody [OTI10H5]
See all TRPM4 primary antibodies -
Description
Mouse monoclonal [OTI10H5] to TRPM4 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Dog, Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human TRPM4 aa 1-1214. Produced in HEK-293T cells (NP_060106).
Sequence:MVVPEKEQSWIPKIFKKKTCTTFIVDSTDPGGTLCQCGRPRTAHPAVAME DAFGAAVVTVWDSDAHTTEKPTDAYGELDFTGAGRKHSNFLRLSDRTDPA AVYSLVTRTWGFRAPNLVVSVLGGSGGPVLQTWLQDLLRRGLVRAAQSTG AWIVTGGLHTGIGRHVGVAVRDHQMASTGGTKVVAMGVAPWGVVRNRDTL INPKGSFPARYRWRGDPEDGVQFPLDYNYSAFFLVDDGTHGCLGGENRFR LRLESYISQQKTGVGGTGIDIPVLLLLIDGDEKMLTRIENATQAQLPCLL VAGSGGAADCLAETLEDTLAPGSGGARQGEARDRIRRFFPKGDLEVLQAQ VERIMTRKELLTVYSSEDGSEEFETIVLKALVKACGSSEASAYLDELRLA VAWNRVDIAQSELFRGDIQWRSFHLEASLMDALLNDRPEFVRLLISHGLS LGHFLTPMRLAQLYSAAPSNSLIRNLLDQASHSAGTKAPALKGGAAELRP PDVGHVLRMLLGKMCAPRYPSGGAWDPHPGQGFGESMYLLSDKATSPLSL DAGLGQAPWSDLLLWALLLNRAQMAMYFWEMGSNAVSSALGACLLLRVMA RLEPDAEEAARRKDLAFKFEGMGVDLFGECYRSSEVRAARLLLRRCPLWG DATCLQLAMQADARAFFAQDGVQSLLTQKWWGDMASTTPIWALVLAFFCP PLIYTRLITFRKSEEEPTREELEFDMDSVINGEGPVGTADPAEKTPLGVP RQSGRPGCCGGRCGGRRCLRRWFHFWGAPVTIFMGNVVSYLLFLLLFSRV LLVDFQPAPPGSLELLLYFWAFTLLCEELRQGLSGGGGSLASGGPGPGHA SLSQRLRLYLADSWNQCDLVALTCFLLGVGCRLTPGLYHLGRTVLCIDFM VFTVRLLHIFTVNKQLGPKIVIVSKMMKDVFFFLFFLGVWLVAYGVATEG LLRPRDSDFPSILRRVFYRPYLQIFGQIPQEDMDVALMEHSNCSSEPGFW AHPPGAQAGTCVSQYANWLVVLLLVIFLLVANILLVNLLIAMFSYTFGKV QGNSDLYWKAQRYRLIREFHSRPALAPPFIVISHLRLLLRQLCRRPRSPQ PSSPALEHFRVYLSKEAERKLLTWESVHKENFLLARARDKRESDSERLKR TSQKVDLALKQLGHIREYEQRLKVLEREVQQCSRVLGWVAEALSRSALLP PGGPPPPDLPGSKD
Database link: Q8TD43 -
Positive control
- WB: HEK-293T cells transfected with pCMV6-ENTRY TRPM4 cDNA; HepG2, HeLa, HT-29, COS-7, Jurkat, MDCK and MCF7 cell extracts. IHC-P: Human kidney, liver, liver carcinoma, ovary and pancreas tissues.
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General notes
Clone OTI10H5 (formerly 10H5).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI10H5 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-TRPM4 antibody [OTI10H5] (ab123936)All lanes : Anti-TRPM4 antibody [OTI10H5] (ab123936) at 1/5000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (Human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (Canine kidney cell line) cell extract
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 134 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)](https://www.abcam.com/ps/products/123/ab123936/Images/ab123936-137366-anti-trpm4-antibody-oti10h5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)Paraffin-embedded human kidney tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)](https://www.abcam.com/ps/products/123/ab123936/Images/ab123936-137365-anti-trpm4-antibody-oti10h5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)Paraffin-embedded human liver tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)](https://www.abcam.com/ps/products/123/ab123936/Images/ab123936-137364-anti-trpm4-antibody-oti10h5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)Paraffin-embedded human liver carcinoma tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)](https://www.abcam.com/ps/products/123/ab123936/Images/ab123936-137363-anti-trpm4-antibody-oti10h5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)Paraffin-embedded human ovary tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)](https://www.abcam.com/ps/products/123/ab123936/Images/ab123936-137362-anti-trpm4-antibody-oti10h5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM4 antibody [OTI10H5] (ab123936)Paraffin-embedded human pancreas tissue stained for TRPM4 with ab123936 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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![Western blot - Anti-TRPM4 antibody [OTI10H5] (ab123936)](https://www.abcam.com/ps/products/123/ab123936/Images/ab123936-137368-anti-trpm4-antibody-oti10h5-western-blot.jpg)
Western blot - Anti-TRPM4 antibody [OTI10H5] (ab123936)All lanes : Anti-TRPM4 antibody [OTI10H5] (ab123936) at 1/5000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY TRPM4 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 134 kDa